Kadavra Tespitinde Kullanılan Formaldehitin Sıçan Karaciğerinde Oluşturduğu Hasar ve Buna Omega-3 Yağ Asitlerinin Etkisi

Objectives: The toxic effects of formaldehyde on liver and protective effects of omega-3 fatty acids against these toxic effects were investigated. Materials and Methods: 21 adult male Wistar rats were divided into three groups. Rats in group I were used as control. Rats in group II were injected with formaldehyde every other day. Rats in group III daily received omega-3 fatty acids with injection of formaldehyde. At the end of 14- days experimental period, all rats were killed by decapitation. The liver tissue specimens which were taken from the rats were examined in light microscope with passing on the routine histological procedures. The activites of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), xanthine oxidase (XO) and malondialdehyde (MDA) were determined in the liver specimens by using spectrophotometric methods. Results: In rats whom formaldehyde was given a statistically significant increase in the levels of SOD, CAT, GSH-Px, XO and MDA was determined when compared to control group. In the light microscopic examination of this group, mononuchlear cell infiltration on portal area and araund central ven was determinated. There were hiperchromatic nucleus and stoplasmic vacuolization in hepatocytes. It was seen that the histological variances which caused by formaldehyde exposure in rats were decreased whom received omega-3 fatty acids. A decrease of SOD, CAT, GSH-Px, XO, MDA levels were determined. Conclusion: It was determined that damage occured in liver tissue of rats exposured to formaldehyde and this damage was reduced by omega-3 fatty acids.©2005, Firat Universitesi, Tip Fakultesi