Method for the detection and determination of viral load of human papillomavirus.

Method for the detection and determination of viral load of human papillomavirus. A pair of degenerate primers capable of amplifying a fragment of the L1 gene from various genotypes of human papillomaviruses (HPV) and a method of detection and quantification of HPV viral load in biological samples isolated, comprising: extracting DNA an isolated biological sample, contacting the sample with a reaction mixture containing at least one fluorochrome and the degenerate primers of the invention amplify a fragment of HPV L1 gene, measuring the fluorescence obtained in the amplification step and compare the fluorescence value obtained to a control sequence.