Analysis on chemical constituents of ershen pill by HPLC-TOF/MS

Objective To analyze chemical constituents of ershen pill by rapid-resolution(high performance) liquid chroma- tography-time of flight mass spectrometry(HPLC-TOF/MS).Methods A shelloseido column(3.0mm×100mm,3μm) was used to separate.The mobile phase consisted of water containing 0.1%methane acid and acetonitrile was used as gradient elute.The flow rate was 0.6 ml/min.TOF/MS was applied for qualitative analysis under positive ion mode.Results Under LC/MS condition,21 major constituents in ershen pill were identified by time of flight mass spectrometry,among which,15 constituents were from Psoralea corylifolia L,and 6 constituents were from Myristica fragrans Houtt.Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of ershen pill.