Abstract 18394: Heat Shock Protein Inducer Geranylgeranylacetone (gga) Protects Against Atrial Fibrillation via the PI3K Pathway

There is evidence suggesting that heat shock proteins protect against clinical atrial fibrillation (AF) by mediating cellular stress. Recently, investigators reported that the HSP-inducing compound GGA prevents the induction of atrial remodeling by AF and attenuates the promotion of AF. An important factor in AF promotion is that atrial cardiomyocytes have constitutively active acetylcholine-activated K+ (IKACh) current, which is enhanced by tachycardia. However, it is whether GGA regulates IKACh remains unclear. We examined the effect of GGA on IKACh current in mouse atrial cardiomyocytes and in an in vitro atrial cell line (HL-1) model of tachycardia remodeling. Methods: In the present study, the IKACh current was recorded using a nystatin-perforated whole cell patch-clamp technique following activation by acetylcholine (10 μM for 2 min). After the measurement of baseline IKACh current, cardiomyocytes were treated with GGA 10 μM for 10 min and IKACh current was re-measured. HL-1 cells were tachypaced vi...