related to Fig 4).

Actin expression and subcellular localization in gut and genitalia organizer is not affected upon DAAM depletion. Actin expression in the larval hindgut LR organizer cells (H1, white bracket), and hindgut precursors (H2, yellow bracket) domain, or larval discs LR organizer cells (A8) in control and DAAM RNAi condition. Actin is detected using FITC-conjugated Phalloidin and gut and genitalia organizer cells are visualized with the expression of RFP under the control of the myo1D-Gal4 driver. Images on the right are cropped single z-plane images corresponding to the white rectangle region(s) on the left panel. Single z-plane images for the gut show lateral views of the cell with on their basal side (bottom of the image) the intensely stained muscular sheet. For quantification of the Actin staining signal in control and DAAM RNAi condition, maximum signal intensity in regions correspond to white and yellow lines in single z-plane images were obtained, then a signal intensity ratio is calculated as an “myo1D-Gal4-positive cell signal (white line)/myo1D-Gal4-negative cell average signal (yellow lines)”. Significance for difference between conditions is assessed with a T-test. p-value is indicated on the brackets showing compared conditions. Threshold for significance of the difference between compared genotypes is defined as: *: <0.05; **: <0.01; ***: <0.001. ns: non-significant. (PDF)