A psychrophilic strainActinoplanes sp. 220

The strain designatedActinoplanes sp. 220 differed in its characteristics from other strains of the genusActinoplanes listed in Sergey’s Manual (1974). The strain belongs to psychrophilic culture growing within the range of 0–30 °C. The optimal temperature for growth on yeast —mali agar is 10–23 °C. Cultures transferred at 23 and 28 °C differed in morphological and physiological properties, enzyme activity and pigmentation in standard media. Submerged culture transferred at 28 °C inhibited growth ofBacillus subtilis ATCC 6633 and ATCC 9945.ll-2,6-Diaminopimelic acid was chromatographically detected in the submerged mycelium of this culture. This compound was not found in the mycelium of the original culture transferred at 23 °C. The cultures did not substantially differ in the composition of other amino acids contained in larger quantities in the mycelium.