The effect of human and rat erythrocyte volume on the structure of hemoglobin protoporphyrin

Changes of erythrocyte hematoporphyrin at different cell volumes were investigated using resonance Raman spectroscopy. Correlation between changes of the position and intensity ratio of 1260 cm-1 bands RRS spectrum and cell volume was observed. Changes of this kind suggest that electronic density redistribution occurs between the C=C and C-N bonds of porphyrin molecule. The redistribution of the electronic density can cause extension of the "core" and increase of the distance between the nitrogen and iron atoms. The iron atom displaces to the macrocycle plane causing decrease of its spin number.