Isothermal amplication rapid detection method of H7N9 avian influenza virus

The invention discloses a method for detecting H7N9 subtype avian influenza virus through loop-mediated isothermal amplification (LAMP). The method is used for detecting avian influenza virus in the fields of foods and raw materials, environmental samples, medical samples and health and disease prevention. According to the technical scheme, the method is characterized in that a specific primer is designed with regional gene sequences respectively coded as HA and NA in an avian influenza genome serving as target sequences, and a reaction system is optimized, so as to perform target gene specific amplification. The method only needs a constant-temperature device, does not need the thermal denaturation and long-term temperature cycle of a template, realizes the direct observation of the results, and has the characteristics of being low in cost, high in efficiency and simple in operation. The LAMP detection method of the H7N9 subtype avian influenza virus nucleic acid, created by the invention, has the characteristics of being high in specificity, high in sensitivity and convenient and quick, can be carried out in a grass-roots level or a small test site, and brings a novel technology and method for detecting the H7N9 subtype avian influenza virus.