Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench

Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homo­ genization of roots with liquid nitrogen, extraction in 0.01 mol L -1 phosphate­buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was mea- sured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS­PAGE gel electrophoresis. The Bradford assay re- vealed that the highest concentration of proteins in frac- tions collected after gel filtration chomatography was 4.66- 6.07 mg mL -1 . Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodula- tory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient.