Quantification of 1,5-anhydro-d-glucitol in urine by automated borate complex anion-exchange chromatography with an immobilized enzyme reactor

Abstract HPLC using a borate form of a strongly anion-exchange resin column and an immobilized enzyme reactor for colorimetric detection was used to quantify urinary 1,5-anhydro- d -glucitol. Urine samples were introduced into the system every 7 min without any pretreatment, and after separation of interfering substances in the column, 1,5-anhydro- d -glucitol was successively detected. Quantitative determination of urinary 1,5-anhydro- d -glucitol was possible within the 1.2–300 μmol/l range. The coefficient of variance was less than 3% and the correlation between results obtained with our system ( y ) and those obtained by gas chromatography–mass spectrometry ( x ) was y =0.983 x −1.287 μmol/l ( n =42, r =0.998).