活体小鼠脑组织Ca(上标 2+)敏感荧光染料双光子成像的观察

To observe the brain image and the change of Ca (superscript 2+) level in live mouse by using two-photon laser scanning confocal microscope. Using craniotomy to expose target area of mouse brain (somatosensory cortex) then injectCa (superscript 2+) sensitive fluorescent indicator, for instance, Oregon Green 488 BAPTA-1 acetoxymethyl. After that, depose the mouse under the two-photon to observe and record. The brain image record by two-photon is clear and vivid .And photograph indicate that by using two-photon confocal can reach deeper area of the brain and record the fast change of Ca (superscript 2+) in single neuron. The results show that by using multi-photon confocal microscope couple with calcium sensitive fluorescent indicator can get high resolution image of brain of live mouse.