Establishment of the Rat Gastric Hot Model

Objective: To establish rat gastric hot model for relevant Chinses medicine pharmacological study.Methods: The rat gastric hot model was established by oral administration with red pepper and alcohol mixture for two weeks and irritated for three days by clipping tails.The temperature,amount of food,water,urination and defecation of every rats were detected by the help of metabolism cage.The content of IL-8,IL2,INF-α,TXB_2,6-keto-PGF1α in blood of rat was examined.Meanwhile,the injury extent of gastric mucosa and the histological characteristics of gastric mucosa were observed.Results: Two weeks after modeling,the model rats had the symptoms of temperature,weight and defecation decreecing and thirst for drinking.Congestion in gastric mucosa occurred and the blood level of IL8,IL-2,(INF-α,)TXB_2,6-keto-PGF1α were higher than those in the control group.However,in model rats pretreated with Zuojin pills,the symptoms and signs of hot-syndrome and histological changes were relieved.Conclusion: Under the controlled condition,feeding rats with red pepper and alcohol mixture combined with clipping tails can be successfully used to establish rat gastric model of hot-syndrome.