Detection of 5 serum cytokines in children with brain tumors by high throughput liquid chip technique

OBJECTIVE: To explore the levels of serum interleukin(IL)-6,IL-8,IL-10,tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ) in children with brain tumors and correlations among them,and their association with the WHO tumor grade.METHODS:Seventy-three children(5-15 years old) with brain tumor and forty-two healthy controls(5-15 years old) were included.Serum levels of IL-6,IL-8,IL-10,TNF-α and IFN-γ of the two groups were measured by high throughput liquid chip technique.RESULTS: Serum levels of IL-6,IL-8,IL-10,TNF-α and IFN-γ in children with brain tumor were higher than those of control groups(P0.01).There were correlations between cytokines.The level of IL-8 in children with low grade of the tumor was higher than that in high grade of the tumor(P0.05).CONCLUSIONS:The results impliy that the cytokines of IL-6,IL-8,TNF-α and IFN-γ may play role in the pathogenesis of pediatric brain tumors.There are interactions between cytokines.IL-8 can be used as a predictor of grade.