Superovulación con sincronización de la onda folicular y con celo natural en vacas Holstein.

Se comparo el efecto de dos protocolos de superovulacion (sincronizacion de la onda folicular SOF y el celo natural CN) en la calidad de embriones obtenidos de vacas Holstein. Se seleccionaron 20 vacas Holstein como donantes con una condicion corporal de 2,75 a 3,5; edades entre 40 y 60 meses; ciclos normales y que no presentaran problemas reproductivos. Las vacas fueron aleatoriamente asignadas al tratamiento SOF (n = 10) y al tratamiento CN (n = 10). El grupo SOF se sincronizo con dispositivo intravaginal de progesterona mas benzoato de estradiol en el dia 0 y FSH en dosis decrecientes durante 4 dias, 2 veces al dia a partir del dia 4. En el dia 6 se retiro el implante y se aplico prostaglandina. En el dia 8 se insemino a las 6 am y 6 pm. Al grupo CN se aplico la FSH dos veces al dia durante 4 dias en dosis decreciente a partir del dia 10. En el dia 12 se aplico prostaglandina y se insemino el dia 14 a las 6 am y 6 pm. En los dos grupos se recuperaron los embriones en forma no quirurgica, 7 dias despues de la primera inseminacion artificial. Los resultados en embriones transferibles fueron 5,7 + 0,76 y 2,8 + 0,31 (P < 0,05) para SOF y CN, respectivamente. Se concluye que el protocolo de SOF produce mayor cantidad de embriones transferibles (calidad 1 y 2) que el protocolo CN.Superovulation with Synchronization of Follicle Wave and Naturally-Induced Estrus in Holstein CowsABSTRACTThe objective was to evaluate the effect of superovulation (SOV) with synchronization of ovarian follicular wave (SOFW) and natural estrus (NE) on the quality of embryos obtained in Holstein cows. 20 Holstein cows were selected as donors with a body condition score: 2.75 to 3.5; aged between 40 and 60 months; normal cycles; and who have not submitted reproductive problems. The cows were randomly assigned to treatment SOFW (n = 10) and NE (n = 10) treatment. The SOFW group was synchronized with intravaginal progesterone device plus estradiol benzoate at day 0 and FSH in decreasing doses for 4 days, 2 times a day from day 4; at day 6 the implant was removed and applied prostaglandin; on day 8, they were inseminated to 6:00 a.m. and 6 pm. At NE group, FSH was applied twice daily for 4 days in decreasing doses from day 10; prostaglandin on day 12 was applied and inseminated on day 14 at 6 am and 6 pm. In both groups embryos non-surgically seven days after the first IA recovered. The results obtained in transferable embryos were 5.7 + 0.76 and 2.8 + 0.31 (P < 0.05) SOF and CN, respectively. It is concluded that the protocol SOFW produces more transferable embryos (grade 1 and 2) that the protocol NE.