Oligomerization analysis at 200 μg/mL concentration of rPF4.

200 μg/mL of rPF4 was subjected to DLS measurements. Only C and D conditions were supplemented with 5 units/mL of UFH. All the conditions were incubated overnight. Conditions A and C were stored at 25°C, whereas conditions B and D were stored at 37°C. Very large complexes of 600 nm to 1200nm were formed between UFH and rPF4 tetramers. Storing at 37°C appears to induce a larger complex formation than 25°C. (DOCX)