Use and methods to prevent and / or treat oral malodor

2009 
inactivates a microorganism belonging to the genus Lactobacillus form, selecting the microorganism from the group consisting of microorganisms having the registration number DSMZ DSM 19825, DSM 19826 or DSM 19827, wherein the inactivated form means a dead cell or inactivated longer can form a single colony on a specific plate for microorganisms belonging to genus Lactobacillus in which said dead or inactivated cell has a cell either intact or ruptured membrane and the microorganism shows the following property (b) when subjected to the following tests (a): Test (a): (a) the microorganism is cultured for 24 37C under anaerobic conditions in a synthetic medium containing peptides 15 g / l with an initial cell density of 1x107 10 cells / ml, in which the synthetic medium has the following composition: guanine: 0.1 g / l cytosine: 0.1 g / l thymidine: 0.1 g / l 2'-deoxyadenosine 0.1 g / l 2'-deoxy uridine: 0.1 g / l K2HPO4: 2 g / l sodium acetate 5 g / l MgSO4 heptahydrate 0.1 g / l diammonium hidrogenocitrato: 2 g / l CaCl2 dihydrate: 0.5 g / l oleic acid 0.1% (w / v) cyanocobalamin 0.02 mg / l riboflavin, 10 mg / l folic acid 0.2 mg / l 5-pyridoxal phosphate monohydrate: 10 mg / l 4-aminobenzoic acid: 0 , 2 mg / l D - (+) - biotin: 1 mg / l ascorbic acid: 500 mg / l nicotinic acid: 10 mg / l Ca pantothenate: 10 mg / l thiamine 1 mg / l of cobalt nitrate (II ) hexahydrate: 500 mg / l MnSO4 monohydrate: 20 mg / l MgSO4 heptahydrate: 500 mg / l Na2MoO4: 0.04 mg / l PTU extract: 15 g / l 7 g / l D-glucose monohydrate: 10 g / l ; (B) the cells are removed by centrifugation at 4000 xg for 15 min; and (c) the peptide concentration in the resulting supernatant is determined; Property (b): the microorganism leads to a reduction of the peptide concentration in the culture medium so that the peptide concentration in the supernatant after incubation for 24 h is reduced by at least 20% compared to the concentration initial 15 g / l, i.e. the microorganism can reduce the peptide concentration in the medium in the assay (a) at least 20%, in which the inactive form shows the property of reducing by at least 20% the peptide concentration of a synthetic medium containing peptides 7 g / l when added to said medium and incubated for 5 min at 37 ° C under aerobic conditions.
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