Sex determination in bloodstains and single hairs

Several methods for sex determination of human DNA have been developed, focusing on detection of the Y-specific chromosomal sequences(Gosden 1984; Fukushima 1988; Nagai 1991). The major component is a repeated sequence family characterised by the presence of Hae III sites spaced at 3.5kb. Various DNA extraction methods and DNA analysis were studied, with the aim of performing sex determination of minute human materials (McCabe 1987).