A facile and sensitive fluorescence assay for glucose via hydrogen peroxide based on MOF-Fe catalytic oxidation of TMB.

Abstract Metal-organic framework (MOF) MOF-Fe nanosols were prepared, which exhibits strongly catalysis of the new fluorescence indicator reaction of 3, 3′, 5, 5′-tetramethylbenzidine (TMB)–H2O2 to produce the oxidation product TMBOX. The TMBOX fluorescent probe has a strong fluorescence peak at 405 nm. After optimizing the various conditions for the determination of H2O2 system and glucose system, the linear range of fluorescence determination of H2O2 was 0.75–7.5 μM, and the detection limit was 0.3 μM. Since H2O2 is the product of glucose oxidase (GOD) catalyzed oxidation of glucose, and a simple and convenient fluorescence method was also established for glucose. The results show that the glucose concentration in the range of 0.2–20 μM has a good correlation with the fluorescence intensity, and the detection limit of glucose was 0.1 μM. This method has been used to detect the content of glucose in drinks with satisfactory results.