Detection of bacterial endotoxin in creatine phosphate sodium for injection

The feasibility for detecting the bacterial endotoxin in creatine phosphate sodium for injection via tachypleus amebocyte lysate test was evaluated.Using the tachypleus amebocyte lysate with sensitivity of 0.5 EU/mL,the experiment was carried out to study the interference effect of sample's concentration on the congregating reactivity between tachypleus amebocyte lysate and bacterial endotoxin.The results showed that samples had no inhibitive or enhanced effect on the congregating reactivity when sample's concentration was 2.0 mg/mL,indicating that the method of tachypleus amebocyte lysate test for detecting the bacterial endotoxin in creatine phosphate sodium for injection is simple,rapid and reliable.