Analysis & Trouble Shooting of the Contaminations, Arised in North Indian Zones during Plant Tissue Culture

In the present investigation Jatropha and pea plant leafs was used, to germinated the plant by artificial condition through plant tissue culture method. We have taken three methods Method A, B & C to germinate callus, seed, bud and stem. 30 Sample were taken to analyze. In Method A 60% samples were contaminated (fungus and bacteria) and 20% was shown growth. In Method B 66% was contaminated by fungus and 19% were contaminated by bacteria and only 15% are shown growth. In Method C 85% samples were contaminated by fungus and bacteria, only 15 % samples was show growth. The study shows most of all contamination was due to Fungus, a very less contamination were due to bacteria. Among three method used with different Sterilization process. Method A was best suited for Callus Culture, Method B for Seed Culture & Method C for Stem & Bud Culture.