Bacterial Community Structure in Different Eco-restoration Areas in a Eutrophic Lake Based on 16S rDNA-DGGE and FDC

Water samples were collected in different ecological restoration areas in Feb of 2005, and genomic DNA was extracted by using SDS-CTAB. The PCR amplification of the extracted microbial 16S rDNA gene fragments was done using primers GC341F and 518R. The result of agarose gel (1.2%) electrophoresis showed that the PCR products were about 230 bp in length. Using PCR products, DGGE was performed on a DGGE analyzer with 8% (w/V) loading acrylamide (38:1, acrylamide:bisacrylamide) gel under 35%~55% linear gradient of denaturant, and the abundance of bacteria was observed by epiftuorescence direct counting. The results showed that both the bacterial community and abundance changed in different ecological restoration areas. After the implementation of engineering measures, the community diversity of bacteria increased, and the main bands of DGGE representing the diversity of bacterial species added 12 from un-restoration areas to eco-restoration areas. However, the abundance of bacteria decreased from 5.36×106 cells/mL to 2.06×106 cells/mL. After two years′ ecological restoration, the water quality was improved obviously, which made the bacterial community diversity increased. Fig 6, Tab 1, Ref 40