Production of a monoclonal antibody to rice stripe virus and research of its characteristics.

One cell strain by hybridoma bridoma cell linesecreting monoclonal antibodies(McAbs) against RSV was produced by fusing mouse myeloma cells(SP2/0) with spleen cells from BALB/c immunized by the purification RSV particles,which was named 1HZ.We prepared ascite fluids types of McAbs with 1HZ.Based on 1HZ,a McAb against RSV was developed,and the titer of the McAb was 1∶1.024×106,when tested by indirect ELISA.The McAbs didn't cross-react with proteins extracted from Oryza sativa,Niaparvata lugens(Stl),Laodelphax stratells and sogatella furcifera(Horvath) virus.Dectection of sensitivity was antigen protein 1: 2.14×104 dilution,and corresponding antigen chroma was 25.89 μg·L-1.The monoclonal antibody 1H2 was purified by saturated(NH4)2SO4.The content of IgG was 6.05 g·L-1.The results suggest that the McAb antibody could be used to detect the dynamic of RSV in the field.