Production of a monoclonal antibody to rice stripe virus and research of its characteristics.
2010
One cell strain by hybridoma bridoma cell linesecreting monoclonal antibodies(McAbs) against RSV was produced by fusing mouse myeloma cells(SP2/0) with spleen cells from BALB/c immunized by the purification RSV particles,which was named 1HZ.We prepared ascite fluids types of McAbs with 1HZ.Based on 1HZ,a McAb against RSV was developed,and the titer of the McAb was 1∶1.024×106,when tested by indirect ELISA.The McAbs didn't cross-react with proteins extracted from Oryza sativa,Niaparvata lugens(Stl),Laodelphax stratells and sogatella furcifera(Horvath) virus.Dectection of sensitivity was antigen protein 1: 2.14×104 dilution,and corresponding antigen chroma was 25.89 μg·L-1.The monoclonal antibody 1H2 was purified by saturated(NH4)2SO4.The content of IgG was 6.05 g·L-1.The results suggest that the McAb antibody could be used to detect the dynamic of RSV in the field.
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