Effects of acute and continuous heat exposure on expression of heat-shock related genes in mice skin.

【Objective】 The study was done to investigate the effects of acute and continuous heat exposure on expression of heat-shock related genes in mice skin in order to explore the molecular mechanism of heat injury.【Method】 Animals were placed in an oven with different temperatures preestablished(43-61 ℃ with an interval incremental of 2 ℃) to receive acute and continuous heat exposure until death,and the control was in the room temperature condition.Immediately after dissecting the dead mice,the mice skin was quickly rinsed with 0.1% DEPC water and saved in liquid nitrogen before use.RT-PCR was then used to examine the heat-shock related genes expression in mice skin followed with image analysis.【Result】 Results showed that expression of hsp70,krt6α and thbs1 was significantly upregulated in the temperatures examined compared with the control.The upregulation of hsp70 affected by the temperatures for exposure showed a dynamic pattern but not for krt6α and thbs1.In short,the upregulation of hsp70 was extremely rapid when the temperatues were below 55 ℃ but slow when above 57 ℃.In addition,compared with the control,the upregulation of hsp105 could be observed in relatively lower temperatures(51 ℃ and below) but not in higher temperatures(53 ℃ and above).Therfore,there were also certain relations with acute high heat and acute low heat.【Conclusion】 In conclusion,our data suggested that acute and continuous heat exposure significantly influenced the expression of heat-shock related genes in mice skin,while the expression patterns of different genes were largely related with the temperatures during heat exposure.Additionally,hsp70 might be considered as a biomarker to quantify the degrees of heat exposure injury in the body.