Laser Biotechnology for Enhanced Rooting and Shooting of Salix Viminalis in Hydroponic Condition for Better Adaptation in Industrially Contaminated Land

Laser Photo stimulation of selected plant and microbial species has been very important in reclamation of several degraded land masses as well as effective treatment of waste and contaminated water sources. The experiment on Laser Photo-stimulation of different size of willow cuttings is carried out in laboratory condition at Department of Environment protection Engineering of AGH-UST, Krakow, Poland. Laser irradiation with selected wavelength and time of exposition of willow cuttings and establishment in hydroponic culture system resulted in enhanced development of roots and shoots compared to the non-irradiated control group. Experimental observation made every week after the irradiation has results of increased number of roots and sprouts. Root buds also increased rapidly in number after second week. Roots changed into highly dispersed secondary roots in II and IV experimental groups and sprouts also started to develop leaves after second week. Remarkable differences in control and experimental groups were seen after five weeks of hydroponic culture. The enhanced development of roots, shoots and other protective organs like root caps after laser irradiation is supposed to help willow species to grow fast and adapt in industrially contaminated land.