Polymorphism of Duffy blood group gene in Shenzhen blood donors

Objective To establish molecular method for Duffy blood group genotyping,and to study the polymorphism of Duffy blood group gene in Shenzhen blood donors.Methods A total of 900 blood samples from unrelated blood donors were phenotyped for Fya and Fyb by the antiglobulin gel test.PCR-SSP and direct DNA sequencing of the FY gene coding region were established.All of them were detected by PCR-SSP.Fifty DNA samples were studied by direct DNA sequencing.Results A total of 855 of them were Fy(a+b-),43 of them were Fy(a+b+),and 2 of them were Fy(a-b+),respectively.And Fy(a-b-) had not been found in our study.The serological results were completely in accordance with FY genotypes by PCR-SSP.The fifty samples sequencing results were consistent with the results via serology and PCR-SSP.The results of sequencing showed the individuals in Fy(a+b-) and Fy(a-b+) phenotype differed by a single substitution at nt 125GA in exon 2 of FY gene.Conclusion DNA-based PCR-SSP and direct DNA sequencing can be correctly genotyped for FY gene.It will be useful in the polymorphism of FY gene research.Fya gene frequency was 0.973 9,Fyb gene frequency was 0.026 1 in Shenzhen blood donors.