ABSORPTION CORRECTION METHOD FOR THE SIMULTANEOUS ESTIMATION OF N-ACETYL- L-CYSTEINE AND AMBROXOL HYDROCHLORIDE IN BULK AND IN COMBINED TABLET DOSAGE FORM Original Article

Objective: The objective of this research was to estimate the concentrations of N-Acetyl-L-cysteine (NAC) and Ambroxol Hydrochloride (AMB) simultaneously, in bulk and combined tablet formulation using a new, simple, precise and accurate absorption correction method using UV Spectrophotometer. Methods: The concentrations of both the drugs (NAC and AMB) were determined using absorption correction method as; at 244 nm only AMB gave substantial absorbance and at 220 nm both NAC and AMB gave absorbance. Distilled water was used as a common solvent for both the drugs and the method was developed. Further statistical evaluations were carried out and the method was validated. Results: In the range of 3-18 µg/ml for AMB at 244 nm and 20-120 µg/ml for NAC at 220 nm, the Beer’s law was obeyed. Percentage recovery for AMB was in the range 100.50-101.10% and for NAC it was 99.85-100.20%. The % RSD values reported were less than 2. The developed method was validated and was found to be linear, accurate, precise and also rugged. Conclusion: The results obtained clearly demonstrated that the proposed method of analysis was simple, sensitive, accurate, precise, rapid and also economical and could be applied successfully for the simultaneous estimation of NAC and AMB in bulk and combined tablet formulation.