Detection of Histone Modifications Associated with miRNAs

: The posttranslational modifications of histones and miRNAs are key epigenetic mechanisms participating in the development, growth, and reproduction of plants. Recently, coordination between these two mechanisms has been demonstrated; each mechanism can be controlled by the other for the regulation of several biological processes. For example, the acetylation of histone H3, a key modification for chromatin remodeling and gene activation, has been linked to the actions of miRNA. In this work, we describe a method for the isolation and immunodetection of two posttranslational modifications in the residues of lysine 9 and 27 of H3, which have been associated with long miRNAs in plants.