Analysis of L1 gene polymorphism of human papillomavirus type 52 from Chaozhou area of China

Objective Purpose To study the polymorphism of L1 gene of human papillomavirus(HPV)subtype 52 in Chaozhou area,and provide the useful data for designing and applying of L1 gene relied vaccine.Methods HPV-52 was detected by HybriMax.The DNA was extracted from the cervical exfoliated cells of 101 cases of simple infection of HPV 52.The complete L1 gene sequence was amplified with four pairs of primers,and then sequenced in Invitrogen Biotechnology Co.Ltd.The BLAST 2.0 software online were used to analyze and identify mismatches.Results Totally,73 complete L1 genes were successfully amplified and sequenced.The similarity was 99%compared with reference strain(GQ472848).31 nucleotide acids were variable,25 of them were nonsense mutation,the other 6 were sense mutation.All of 73 cases were divided into East Asian and Europe lineage according to the polymorphism of L1 gene.East Asian cluster include 13 variants,CZ52A105 represent the most common variant(61.64%,45/73),this variant was named "Chaozhou isolate",its sequence was completely matched with isolate IN141070(HQ537743)reported in Thailand,while the other 12 variants have never been reported worldwide so far,their sequence was the closest homology with sequence reported in Tianjin TJ49-52(GQ472848)and Thailand IN141070(HQ537743),one to six mismatches was found.Europe cluster included two kinds of variants,which were isolate CZ52E928(2/73)and CZ52E136(5/73),their sequence were completely matched with sequence reported in German REF(X74481) and Guanacaste Qv03719(HQ537745)respectively.Conclusion The most common polymorphism of HPV52 L1 genes was similar with that reported in the south-east China or other south-east Asia countries,CZ52A105 was named the predominant sequence in Chaozhou area.This study provided molecular evidence for gene vaccine exploration in Chaozhou.