Bioinformaticsanalysescombinedmicroarray identifythedesregulatedMicroRNAs inlungcancer

BACKGROUND: MicroRNAs (miR - NAs) play an important role in the regulation of cell growth, differentiation, apoptosis, and car - cinogenesis. Deregulated miRNAs are found in blood cells of cancer patients recently. AIM: This study aims to screen the differen - tially expressed miRNAs (DE-miRNAs) which could discriminate lung cancers from non-can - cerous lung tissues as well as molecular signa - tures that differ in tumor histology. MATERIALS AND METHODS: miRNA expres - sion profiles of GSE17681 was downloaded from Gene Expression Omnibus database. Three test methods were used to identify DE-miRNAs be - tween lung cancer tissue and healthy controls. Target genes of DE-miRNAs were retrieved from three databases and mapped to KEGG to investi - gate their roles in lung cancer. Further, a protein- protein interaction (PPI) network was construct - ed used STRING and Cytoscape. RESULTS: A total of 17 DE-miRNAs were identi - fied. Among them, hsa-miR-339-5p draw specific attention. Pathway analysis revealed that target genes of RASSF1 and KRAS play roles as onco - gene or tumor suppressor gene in the progression of lung cancer. Besides, Target genes of RASSF1 and ERBB4 formed a module in the PPI network. Functional analysis suggested biological process of response to hypoxia was significantly enriched. CONCLUSIONS: hsa-miR-339-5p play impor - tant role in the regulation of lung cancer and it may be potential to be used as biomarker to predict lung cancer progression.