The Pathogen Protein EspFU Hijacks Actin Polymerization Using Mimicry and Multivalency

Enterohemorrhagic E. coli (EHEC) attaches to the intestine through actin pedestals that are formed when the bacterium injects the protein EspFU into host cells1. EspFU potently activates the host WASP (Wiskott-Aldrich syndrome protein) family of actin-nucleating factors, which are normally activated by the GTPase Cdc42, among other signaling molecules. Apart from its N-terminal type III secretion signal, EspFU consists of five and a half 47-amino-acid repeats. Here we find that a 17-residue motif within this EspFU repeat is sufficient for interaction with N-WASP. Unlike most pathogen proteins that interface with the cytoskeletal machinery, this motif does not mimic natural upstream activators: instead of mimicking an activated state of Cdc42, EspFU mimics an autoinhibitory element found within N-WASP. Thus EspFU activates N-WASP by competitively disrupting the autoinhibited state. By mimicking an internal regulatory element and not the natural activator, EspFU selectively activates only a precise subset of Cdc42-activated processes. Although one repeat is able to stimulate actin polymerization, we show that multiple-repeat fragments have dramatically increased potency. The activities of these EspFU fragments correlate with their ability to coordinate activation of at least two N-WASP proteins. Thus this pathogen has used a simple autoinhibitory fragment as a component to build a highly effective actin polymerization machine.