The Effect of Heparin and Small Volume Hypertonic Saline on the Post-resuscitation Intestine Microcirculation Perfusion Disorder of Rabbits

Objective Evaluating the beneficial effect of heparin and small volume hypertonic saline during the cardiopulmonary resuscitation(CPR) on the post-resuscitation intestine microcirculation perfusion disorder of rabbits.Methods Sixty domestic rabbits were anesthetized with embutal,30 mg·kg-1 and Cardiac arrest was induced by electrical ventricular fibrillation,animals randomly received 2 mL·kg-1 of either 0.9% NaCl(NS) or 0.9% NaCl combined with heparin 60 U·kg-1(H) or 7.5% NaCl combined with heparin 60 U·kg-1(HSH) in CPR.Observe each group of small intestinal perfusion area.Ventricular fibrillation ROSC measured before and after plasma sP and ET concentration change.Results After the ROSC,the MAP were no difference in all groups,The area of intestine perfusion was higher in group HSH than the other two groups,the difference was extreme significance(P0.01),respectively.Between the group NS and group H,the difference of intestine perfusion area was also significant.At the time point of ROSC 20 min and ROSC 30 min the plasma concentration of sP was lower in group HSH than the other two groups.ET between the groups no significant difference between the basic value,CA Later,following the restoration of spontaneous circulation,rabbit plasma ET concentration was significantly increased,and with the restoration of spontaneous circulation has gradually increased the time to extend the trend.ROSC 30 min,ROSC 60 min,ROSC 120 min,ROSC 3 hours,ROSC 4 hours when the HSH group,H group of ET were significantly lower than NS group(P0.01);while the HSH group and the H was no significant difference between group(P0.05).Conclusion The administration of heparin and small volume hypertonic saline during the CPR could attenuate the perfusion disorder and reduce the recovery of plasma endotoxin concentration in rabbit,but not increased the risk of bleeding.