UHPLC determination of residues of chlorpyrifos insecticide in chicken meat

The present study was therefore aimed to determine the residual concentration of one of the most commonly used insecticides chlorpyrifos in meat samples of chicken, on the basis of surveillance at various poultry farms in and around Jabalpur district. A total number of 150 meat samples were collected from different poultry farms and local market in and around Jabalpur. The extraction procedure was based on liquid–liquid partition and clean-up procedure by alumina column technique. Acetonitrile and water were used as mobile phase in the ratio of 80:20 (v/v) for UHPLC determination. The limit of detection and limit of quantification for chlorpyrifos was as 0.014 mg/kg and 0.0463 mg/kg, respectively. The recoveries of chlorpyrifos in meat samples found in the range of 82-94 per cent and none of the analyzed samples were found positive for residual concentration of chlorpyrifos in meat samples.