Pharmacokinetic Evaluation of Flavonoid compound (acacetin) Isolated from Gmelina arborea roxb

Aim: The flavonoid compound (Acacetin) isolated from fruits of Gmelina arborea was investigated for its pharmacokinetic evaluation to find out the suitability of this compound to be formulated in any suitable dosage form. Method: The acacetin was administered intravenously and orally in Wistar rats at a dose of 2 and 10 mg/Kg body weight respectively. In a regular interval of specified time, blood samples were collected and bio-analyzed to quantify the drug concentration in the blood sample by using LC-MS. The Cmax, Tmax, T1/2, KE, Ka, and bioavailability (F) of acacetin were determined by mathematically and graphically from plasma concentration-time profile data. Absorption rate constant was determined by the method of residual. Results: From the i.v. Bolus administration data, acacetin had an area under curve (AUC) is 1.542 µg.h/ml, elimination rate constant (KE) is 0.423 h-1, and half-life (T1/2) is two hour. The oral administration of acacetin showed the peak plasma concentration (Cmax) of 1.668 µg/ml, Tmax is 1 h, AUC is 6.44 µg h/ml, KE is 0.416 h-1, T1/2 is 2 h, absorption rate constant (Ka) is 1.6 h and bioavailability of acacetin was found to be 84 %. Conclusion: From the study it could be concluded that the acacetin possessed relatively greater bioavailability; thus this drug exhibited significant satisfactory pharmacokinetic profile which would be helpful for successful designing of a suitable dosage form formulation.