Heteronuclear three-dimensional NMR spectroscopy applied to CMP-KDO synthetase (27.5 kD)
1989
Abstract A heteronuclear three-dimensional NMR experiment has been applied to uniformly 15 N-labeled CMP-KDO synthetase (CTP:3-deoxy-D-manno-octulosonate cytidylyl transferase; E.C. 2.7.7.38) complexed with an inhibitor and CTP. Using this 3D technique, the 2D NOE spectrum of the ternary complex was dramatically simplified by editing with respect to the 15 N frequencies of the labeled enzyme. This 3D NMR method is a useful tool for resolving spectral overlap and is particularly well-suited for NMR studies of large molecules which are difficult to study by conventional 2D NMR techniques.
Keywords:
- Fluorine-19 NMR
- Heteronuclear single quantum coherence spectroscopy
- Nuclear magnetic resonance spectroscopy
- Nuclear magnetic resonance spectroscopy of nucleic acids
- Two-dimensional nuclear magnetic resonance spectroscopy
- Transverse relaxation-optimized spectroscopy
- Stereochemistry
- Nuclear Overhauser effect
- Heteronuclear molecule
- Chemistry
- Biochemistry
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