Proliferation effect of neural stem cell of ginsenoside Rg1 in vitro

Objective:To study the proliferation effect of neural stem cells(NSCs) of ginsenoside Rg1 in vitro.Method:NSCs from the embryonic rat(El4) were isolated,10 mg·L-1 BrdU were added in the medium.The NSCs were incubated together with 1,10 μmol·L-1 ginsenoside Rg1 for 48 hours,control group were setup.Then BrdU positive cell number were detected and counted by fluorescence microscop.The Hes1 and Mash1 mRNA expression were detected by real-time RT-PCR.Result:The number of BrdU positive cells in 10 μmol·L-1 ginsenoside Rg1 group was increased significantly compared with the control group(40.5±10.9 vs 26.2±6.0,P0.01),and the Hes1 mRNA expression were increased significantly(1.00±0.11) vs(1.28±0.14),P0.05.Conclusion:ginsenoside Rg1 can promote the proliferation of neural stem cells in vitro,and this effect may be induced by the up-regulation Hes1 expression.