TSWV resistance in DH lines of tobacco (Nicotiana tabacum L.) obtained from a hybrid between ‘Polalta’ and ‘Wiślica’

With 2 tables Abstract Nicotiana tabacum cv. ‘Polalta’ is reported as having a hypersensitive type of Tomato spotted wilt virus (TSWV) resistance. In ‘Polalta’ hybrids with susceptible cultivars, morphological deformations (thickened and ribbon-shaped leaves, irregular venation, tumours) appear. Acceptable DH lines derived from hybrids of ‘Polalta’ with ‘Wiślica’ were obtained by a method of in vitro culture. Those DH lines were evaluated for TSWV resistance. Tests for resistance were conducted under greenhouse conditions using one TSWV isolate from a local source. The presence of the virus was verified using DAS-ELISA. The DH lines PW-833, PW-834, and PW-900 showed a near correct morphology and stably inherited dominant monogenic resistance to TSWV.