The effect of the specific shRNA of midkine on the proliferation and apoptosis of gastric carcinoma cell BGC823

Objective To investigate the effect of knockdown of midkine(MK) expression by shRNA on cell proliferation and apoptosis of human gastric carcinoma cell BGC823.Methods Specific shRNA plasmids to midkine were constructed,and were then transfected into BGC823 by lipofectamin 2000.The cell proliferation was evaluated by CCK-8 kit,and the apoptosis was determined by flow cytometric analysis.Results The recombinant plasmid expressing midkine shRNA effectively inhibited BGC823 cell proliferation(P0.01) and decreased clone formation(P0.01) and significantly promoted cell apoptosis with the emergence of sub-diploid peak(P0.05).Conclusion shRNA plasmids targeting MK gene can inhibit the growth of human gastric cancer cells by attenuating cell proliferation and inducing apoptosis,which might provide experimental evidence for gene targeting therapy of MK.