Induction on callus culture and regeneration of Orostachyis fimbriatae

Objective:To explore the effects of different hormonal combinations on induction,proliferation and differentiation of Orostachyis fimbriatae callus culture.Methods:Aseptic seedling leaves were used as explants,the different concentrations of IAA,NAA,6-BA and KT on induction proliferation of callus were optimized by orthogonal test to explore the optimum medium for differentiation of callus by tissue culture techniques.Results:The best medium for induction was MS+IAA 1.0 mg/L+NAA 0.5 mg/L+KT 1.0 mg/L,and the best hormonal combination for proliferation was MS+IAA 0.5mg/L+6-BA 0.5 mg/L+KT 1.0 mg/L.The best medium for differentiation was MS+IAA 0.1 mg/L+KT 2.0 mg/L,and 1 /2MS+IAA 0.2 mg/L was the optimum medium for rooting culture.Conclusion:The system of regeneration of Orostachyis fimbriatae is establishd by tissue culture techniques in this study.