TRANSEPIDERMAL WATER LOSS MEASUREMENTS FOR ASSESSING SKIN BARRIER FUNCTIONS DURING IN VITRO PERCUTANEOUS ABSORPTION STUDIES
1999
In the course of performing in vitro percutaneous absorption studies, it is important
to ensure the integrity of the stratum corneum. In vitro assessment of the permeability of chemicals is often preceded by studying the rate of penetration of tritiated
water (1,2). However, this technique has several limitations. For example, it results
in hydrated stratum corneum, possibly affecting the penetration of the compound
under investigation. This method cannot be used if the test compound is tritium
labeled. In addition, it is time-consuming and requires a license and facilities to
handle radioactive materials. In practice, the repetitive measurement of tritiated
water in laboratories is accomplished by determining the fraction of the dose applied
absorbed at a fixed time. Selection of that fixed time is difficult without a priori
knowledge of the permeability, and improper selection can lead to an erroneous
assessment of the barrier properties of skin. Recently an alternate technique, which
measures the electrical resistance across the skin slices, has also been proposed for
this purpose (3). Based on the initial results, this technique appears to have some
advantages, but more studies are required to validate it.
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