Research on Phenotypic Mutations in M_2 Population Derived from EMS Treatment in Brassica napus L.

Seeds of NJ7982(Brassica napus L.)were treated with EMS of four concentrations(0%,0.2%,0.4%,0.6%)and resulted in 0.4% EMS treatment with induction effect better than others.Observation of M2 generation population with 48000 plants showed that there existed mutations for six organs in the population.Cotyledon mutation traits included tricotyledon,yellowish cotyledon,etc,accounting for 0.22% in the population.Leaf mutation traits contained yellowish leaf,albino leaf,purple leaf,up-curly leaf,down-curly leaf,etc,with the mutation frequency of 0.74%.Flower organ mutation traits of 9.38% included purple buds,bud-deads,three petals,six petals,white petal,white-mosaic petal,wrinkled-petal,complete male sterility,partial fertility,etc.Plant type mutation traits of 4.98% included dwarf plant,purple stem,etc.Silique mutants accounting for 2.79% included thick silique,long silique,purple silique,etc,and yellow-seeded mutants accounted for 0.40%.The total mutant frequency was 18.51% in the M2 population.All these mutatied materials are conserved in germplasm library,and will be used in rapeseed functional genomic research and variety improvement.