Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarréia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal (Detection of Brucella spp., Leptospira spp., bovine herpesvirus and bovine viral diarrhea virus nucleic acids in aborted fetuses and bovines dead perinatal)

ABSTRACT Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV) and bovine viral diarrhea (BVDV) in 3 animals (2.4%) each, and 1 for the association of BVDV and BHV. In 77.4 % (96/124) of the samples it was not possible to detect any agent. Keywords: bovine, abortion, fetuses, PCR, RT-PCR AGRADECIMENTOS Ao Prof. Silvio Arruda Vasconcellos, por ceder as amostras-padrao de Leptospira spp. Ao medico veterinario Henrique Leomil, pelo auxilio nas necropsias. REFERENCIAS BIBLIOGRAFICAS CHOMCZYNSKI, P. A reagente for the single step simultaneous isolation of RNA, DNA and proteins froms cells and tissue samples. Biotechiniques , v.15, p.532-537, 1993 CORTEZ, A.; SCARCELLI, E.; SOARES, R.M. et al. Detection of Brucella DNA from aborted bovine fetuses by polymerase chain reaction. Aust. Vet. J . ,