Screening, identification and degradation characteristics of a dominant fluorene-degrading strain.

A dominant strain(named W-2) which can utilize fluorene as carbon and energy source for growth was isolated and screened from activated sludge of a coking plant.On the basis of morphological observation and physio-biochemical test,the strain W-2 was identified as Stenotrophomonas acidaminiphila by 16S rDNA gene sequence and phylogeny analysis.The biodegradation efficiency of fluorene by strain W-2(S.acidaminiphila) in solution culture was further investigated.Results indicated that the strain could effectively degrade fluorene and the biodegradation efficiency reached 86.0%after 11 days under the conditions of initial fluorene concentration 40mg/L,inoculation amount 10%(V/V),pH 7.0 and temperature 30℃.The results showed that strain W-2 had a good application prospect in control of fluorene pollution.