[Long-term drinking purified water may aggravate the inhibition of NMDA expression and spatial learning ability induced by lead on rat].

Objective To compare brain lead acctunulation and neurotoxicity induced by had under drinking purified water and tap water Oil rat.Methods AU 1 04 male weaning SD rats were randomly divided into eight groups,matched-four pairs according to drinking water:tap water,purified water,tap water with lead 50 mg/L(1ead acetate water-solution).purified water with lead 50 mg/L,tap water with lead 200 ms/L.purified water with lead 200 mg/L.tap water with lead 800 mg/L All were fed witlI normal food and endronmental cognitions kept consistent.Morris water maze(including Place Navigation,Spatial Probe Test, Visible Platform Trial)was measured to test rat spatial learning at the 12 and 24 week.At the end of the experiment(28 week),rats were killed and the lead of brain and blood was measured by Graphite furnace atomic absorption spectrometric method;the NRl,NR2A,NR2B 0f NMDAR(N.methyl.D.aspartame receptor)in hippocampus were analyzed by RT-PCR Results Under the sanle lead exposure.nO significant differences were observed in blood lead.however.brain lead level showed hisher in drinking purified water group than that in tap water group.Expression of NRl.NR2A and NR2B in hippocampus of the rats drinking purified water WaS lower than those drinking tap water,especially at low lead exposure(50 ms/L)(P

Keywords:

• Purified water
• NMDA receptor
• Biochemistry
• Biology
• lead exposure
• Tap water
• Animal science
• Weaning
• spatial learning
• Water maze
• Neurotoxicity
• Chemistry

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