[Expression of cytokines Il-6, IL-34 and M-CSFR in chronic periodontitis and its clinical significance].

PURPOSE: To detect the expression of IL-6，IL-34 and M-CSFR in chronic periodontitis and healthy gingival tissue and discuss the role of IL-6，IL-34 and M-CSFR in the etiology of chronic periodontitis. METHODS: A total of 8 patients with mild chronic periodontitis and 8 patients with severe chronic periodontitis were collected in this study. As control，8 normal gingival tissues from extracted healthy were selected. The expression of IL-6, IL-34 and M-CSFR mRNA was detected by real-time PCR; the expression of IL-6, IL-34 and M-CSFR protein was detected by Western blotting. Statistical analysis was performed using GraphPad Prism 6.0. RESULTS: The levels of IL-6, IL-34, M-CSFR mRNA and protein expression in chronic periodontitis was significantly higher than that of the normal gingival tissue(P<0.05). CONCLUSIONS: IL-6, IL-34 and M-CSFR may be closely related to the development of chronic periodontitis.