УСЛОВИЯ СПЕЦИФИЧЕСКОГО СВЯЗЫВАНИЯ МОНОКЛОНАЛЬНЫХ АНТИТЕЛ С АДСОРБИРОВАННЫМИ НА НОСИТЕЛЕ СУСПЕНЗИЕЙ ОПУХОЛЕВЫХ КЛЕТОК
2009
The first procedure of any kind of immunochemical analysis is the identification of be analyzed complex
by specific antibody. The establishing process of «antigen-antibody» in immunochemical complex
is taken under the fixed affinity, concentration of components and stable data relationship of reaction
requirement. The author studied the optimized requirement for indirect ELISA, include determining
of «antigen-antibody» balance constant in immunological complex and evaluation requirement
for combining kinetics with commercial conjugates based on cancer cell surface antigen specific
monoclonal antibodies.
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