Effect of Dachengqi Decoction on Cytokines Tumor Necrosis Inflammatory Cytokines in Rats with Endotoxaemia

Objective:To observe the effect of Dachengqi Decoction(DT)on inflammatory cytokines in rats suffered from the endotoxemia,and to discuss the mechanism of DT when curing the endotoxemia.Method: Fifty SD rats were divided into 5 groups randomly:sham operation group,model group,DT group,Rhei Radix et Rhizome group and Magnolia officinalis group.Two days before model establishment,rats were intervented by intragastric administration(1 g·kg-1)for the medication groups;and 1 hour after the last drug administration,model was established by CLP,then medication was continued for 3 days.The serum content of endotoxin,TNF-α,IL-1β and IL-10 was detected.Result: The content of plasma endotoxin,TNF-α,IL-1β and IL-10 was decreased evidently in curing group compared with those in the model group(P0.01).Concerning IL-1β/IL-10,model group(1.218 6±0.387 9),DT group(0.389 1±0.223 9),Radix Et Rhizome Rhea group(0.366 7±0.248 9),M.officinalis group(0.372 5±0.185 8),each treatment group compared with model group also had significant difference(P0.01).Conclusion: It shows that the mechanism of DT on endotoxemia is related with the antagonistic to the serum inflammatory cytokines TNF-α,IL-1β and the moderating effect to the ratio of IL-1β/IL-10.