Anti-inflammatory and wound healing effects of gel containing Kaempferia marginata extract

Abstract Ethnopharmacological relevance Kaempferia marginata Carey (Zingiberaceae family) has been traditionally used in the treatment of inflammation. The whole plant decoction is used for treatment of fever. Ethanol extracts which exert potent anti-inflammatory properties are selected for wound healing assay. Aim of the study This study aimed to investigate activities of the extract and gel formulation on anti-inflammatory and wound healing activities. The anti-inflammatory and wound healing properties from this plant could support its traditional uses and obtain a new pharmaceutical product with good physical, chemical and biological stabilities. Materials and methods The anti-inflammatory activity was tested on anti-nitric oxide (NO) production using RAW264.7 cells and wound healing assay using human dermal fibroblast (HDF) cells. Results The results found that the anti-inflammatory activity of gel containing K. marginata at 10% w/w showed the highest activity with an IC 50 value of 12.50 μg/ml (Diclofenac gel, IC 50 value = 64.90 μg/ml). It also revealed that wound healing activities of K. marginata gel (5% w/w) showed the highest % cell viability (134.05%) and the highest % cell migration at 85.20 using HDF cells. Conclusion The present study shows that gel containing K. marginata extracts have good anti-inflammatory and wound healing properties in vitro .