Serum pharmacological study of Zilongjin on the inhibition of the proliferation of renal carcinoma cell line ketr-3

Objective To investigate the inhibitory effects of serum with Zilongjin on human renal carcinoma cell line ketr-3.Methods By means of serum pharmacologic method,medicated serum samples containing Zilongjin of different concentrations and human renal carcinoma cell line ketr-3 were co-incubated to observe the morphological changes of the cells at different time points with biological inverted microscopy,and WST-8 assay was used to detect the inhibition of ketr-3 cell proliferation.Results 48 hours after treatment with different concentrations of medicated serum containting Zilongjin,the ketr-3 cells exhibited typical morphologic changes of apoptosis,including evident round shape in some cells,cell shrinkage and appearance of apoptotic bodies;Zilongjin serum could cause inhibition on the proliferation of ketr-3 cells in time-dependent and dose-dependent manners.Compared with the control group,there was no significant difference in the inhibition of cell proliferation for each concentration of medicated serum(5%,10% and 20%) at 24 h(P0.05).At 48 h,the inhibition rate of cell proliferation was 4.9%(P0.05),22.6%(P0.01) and 28.7%(P0.01),respectively.Conclusion Zilongjin-medicated serum can effectively inhibit the proliferation of human renal carcinoma cell line ketr-3 in vitro.