Tyrosinase inhibition of Potentilla bifurca

Objective:To identify the tyrosinase inhibitory constituent quickly from Potentilla bifurca.Method:The active constituent was found through fraction collecting and tyrosinase inhibitory activity by bioassay-linked HPLC method.Result:The methanol extracts and BuOH fraction of Potentilla bifurca showed strong tyrosinase inhibitory activities.From BuOH fraction of Potentilla bifurca,the tyrosinase inhibitory constituent was isolated and identified as flavonoid,quercetin-4'-O-β-D-glucoside.It express stronger tyrosinase inhibition than the known tyrosinase inhibitor,kojic acid(IC50=0.28 mmol·L-1) with IC50 value of 0.001 9 mmol·L-1.Conclusion:Bioassay-linked HPLC fractionation method was provided for determination the active constituents quickly from herbal medicines.