[Determination of 25(R, S) ruscogenin 1-O-[beta-D-glucopyranosyl (1 --> 2)] [beta-D-xylopyranosyl (1 --> 3)] beta-D-fucopyranoside in Liriope muscari from different habitats and different harvest time by HPLC-ELSD].

Objective:To develop an HPLC-ELSD method for the determination of 25(R, S) ruscogenin 1-O-[β-D-glucopyranosyl (1→2)] [β-D-xylopyranosyl (1→3)] β-D-fucopyranoside in the tuberous roots of Liriope muscari from different habitats and different harvest time. Method: A Shimadzu C18 column (4.6 mm×150 mm, 5 μm) with a solvent system consisting of acetonirile-water (46∶54) was used, and detected by ELSD. The temperature of drift tube was 94 oC and the nebulizer nitrogen flow rate was 2.5 L·min-1. Result: The calibration curve of 25(R, S) ruscogenin 1-O-[β-D-glucopyranosyl (1→2)] [β-D-xylopyranosyl (1→3)] β-D-fucopyranoside showed good linearity in the range of 1.02-12.228 μg and the average recovery was 100.80%,with RSD of 1.8%. 10 batches of L. muscari from different habitats were analyzed, and the contents were 0.25%-0.41%. The contents of 15 batches from different harvest time were 0.13%-0.38%. Conclusion: The method is simple, rapid and sensitive, and can be used for determination of 25(R, S) ruscogenin 1-O-[β-D-glucopyranosyl (1→2)] [β-D-xylopyranosyl (1→3)] β-D-fucopyranoside in L. muscari. It provides the valuable basis for quality assessment of L. muscari.